Investigation of Genetic Variations among Crested Wheatgrass Species Base of Agronomical Traits and Total Leaf Protein

Document Type: Research and Full Length Article


1 Research Institutes of Forests and Rangelands, Tehran

2 Payam Noor University of Tehran, Tehran,


Genetic variations within the species of Agropyron desertorum and 2 varieties of A. cristatum subsp. pectinatum var. imbricatum and A. cristatum subsp. pectinatum var. pectinatum were studied using morphological traits and total protein profiles (with sodium dodecylsulphate polyacrylamide gel electrophoresis). An experiment was conducted in Research Institute of Forests and Rangelands (2012-2013) using a completely randomized block design with three replications. Means comparison of different agronomical traits among different wild populations of three taxa showed that A. desertorum had higher plant crown, plant height, panicle length, number of stems and plant dry matter weight; however, var. pectinatum had higher panicle length, grain yield and 1000-grain weight and got into the pollination and full flowering phases in a shorter period. The results of total protein profiles revealed that the genetic diversity of var. imbricatum samples was considerably higher than var. pectinatum and/or A. desertorum. Classification analysis of different populations of taxa using both markers showed that populations of var. pectinatum were closer to A. desertorum than var. imbricatum. The results demonstrated that the study of genetic diversity and the relationships among the crested wheatgrass species using agronomical traits and total protein profiles provides important information for the collection, genetic conservation and planning of future breeding programs.


    Literature Cited

Angell, R. F., Miller, R. F. and Haferkamp. M. R., 1990. Variability of crude protein in crested wheatgrass at defined stages of phenology. Jour. Range Manage. 43: 186–189.

Arghavani, A., Asghari, A., Shokrpour, M. and Chmanabad, M., 2010. Genetic diversity in ecotypes of two Agropyron species using RAPD markers. Res. Jour. Environ. Sci. 4: 50–56.

Asay, K. H., Jensen, K. B., Hsiao, C. and Dewey, D.R., 1992. Probable origin of standard crested wheatgrass, Agropyron desertorum Fisch. ex Link, Schultes. Canadian Jour. Plant Sci. 72: 763–772.

Asghari, A., Jafari, A. A., Shokrpour, M., Mohammaddoust, E. and Chamanabad, H.R., 2011. Genetic variation between and within populations of Agropyron Gaertn. using RAPD Markers. Iranian Jour. Rangelands For. Plant Breed. Genetic Res. 18: 143–153. (In Persian).

Asri, Y., 2011. Range plants of Iran, 1: monocotyledons. Publication of Research Institute of Forests and Rangelands, 573 p.

Assadi, M., 1995. Meiotic configuration and chromosome number in some Iranian species of Elymus L. and Agropyron Gaertner (Poaceae). Bot. Jour. Linn. Society, 117(2): 159-168.

Assadi, M. and Runemark, H., 1995. Hybridization, genomic constitution and generic delimitation in Elymus (Poaceae, Triticeae). Plant Syst. Evol. 194: 189-205.

Barkworth, M. E., Campbell, J. J. N. and Salomon, B., 2007. Agropyron. In: Barkworth, M.E., Capels, K.M., Long, S., Anderton, L.K. and Piep, M.B. (eds.) Flora of North America, Volume 24, Poaceae, part 1, pp. 277-279. New York: Oxford University Press.

Bor, N. L., 1970. Gramineae, in Rechinger, K. H. (ed.) Flora Iranica, no. 70. -Akademische Drueck-U, Verlagsanstalt, Graz, Austria.

Buhteeva, A. V., Malyshev, L. L. and Konarev, A.V., 1990. Wild-growing populations of wheat grass from Eastern European steppe province. Bull. VIR. Leningrad 198: 54–59.

Cardeña, R., Oropeza, C. and Zizumbo, D., 1998. Leaf proteins as markers useful in the genetic improvement of coconut palms. Euphytica, 102: 81–86.

Che, Y. H. and Li, L. H., 2007. Genetic diversity of prolamines in Agropyron mongolicum Keng indigenous to northern China. Genet. Resour. Crop Evol. 54: 1145–1151.

Das, S. and Mukharjee, K. K., 1995. Comparative study on seed proteins of Ipomoea. Seed Sci. Tech.  23: 501–509.

Dewey, D. R., 1983. Historical and current taxonomic perspectives of Agropyron, Elymus and related genera. Crop Sci, 23: 637-642.

Dewey, D. R. and Asay, K. H. 1975. The crested wheat grasses of Iran. Crop Sci. 15: 844-849.

Excoffier. L., 1995. AMOVA 1.55 (Analysis of Molecular Variance). University of Geneva, Switzerland, Genetics and Biometry Laboratory.

Excoffier, L., Smouse, P. and Quattro, J., 1992. Analysis of molecular Variances among DNA restriction data. Genetics, 131: 479–491.

Farshadfar, M. and Farshadfar, E., 2004. Genetic Variation Among Different Agropyron Species Based on Morphological and Chemical Indices. Jour. Water Soil Sci. Isfahan Univ. Tech. 8: 243–251.

Garcia, P., Monte, J. V. Casanova, C. and Soler, C., 2002. Genetic similarities among Spanisch populations of Agropyron, Elymus and Thinopyrum, using PCR-based markers. Genet. Res. Crop Evol. 49: 103-109.

Ghafoor, A., Ahmad, Z., Qureshi, A.S. and Bashir, M., 2002. Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS PAGE. Euphytica, 123: 367–378.

Gower, J. C., 1966. Some distance properties of latent root and vector methods used in multivariate analysis. Biometrika, 53: 325–338.

Hirano, H., 1982. Varietal differences of leaf protein profiles in mulberry. Phytochemistry, 21: 1513–1518.

Hormaza, J. I., Pinney, K. and Polito, V. S., 1998. Genetic diversity of pistachio (Pistacia vera, Anacardiaceae) germplasm based on randomly amplified polymorphic DNA (RAPD) markers. Econ. Bot. 52:78–87.

Huber-Sannwald, E., Pyke, D. A., Caldwell, M. M., 1996. Morphological plasticity following species-specific recognition and competition between two perennial grasses. American Jour. Bot. 83: 919–931.

ISTA, 1993.International rules for seed testing. Seed Sci. Technol. 21: 288.

Jafari, A. A., Seydemohammadi, A. R., Abdi, N. and Areffi,  H. M., 2008. Seed and hay production in 31 genotypes of desert wheatgrass (Agropyron desertorum) using drought tolerance indices. Iranian Jour. Range Desert Res.  15: 114–128. (In Persian).

Jensen, K. B., Larson, S. R., Waldron, B. L. and Asay, K.H., 2006. Cytogenetic and molecular characterization of hybrids between 6x, 4x, and 2x ploidy levels in crested wheatgrass. Crop Sci, 46: 105-112.

Kamel, E. A. and Hassan, L. M. A., 2001. The significance of cuticular features, petiole anatomy and SDS - PAGE in the Taxonomy of the Lauraceae. Pakistan Jour. Biol. Sci. 4: 1094–1100.

Khan, M. K., 1990. Production and utility of chickpea (Cicer arietinum L.) in Pakistan. Progressive Farming, 10: 28–33.

Knowles, R. P., 1955. A study of variability in crested wheatgrass. Canad Jour. Bot. 33: 534-546.

Laemmli, U. K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680–685.

Laghetti, G., Pienaar, B. L., Pasdulosi, S. and Perrino, P., 1998. Ecogeographical distribution of Vigna savi in southern Africa and some areas of the Mediterranean basin. Plant Genet. Resou. Newsletter, 115: 6–12.

Love, A., 1984. Conspectus of the Triticeae. Feddes Repertorium, 95: 425–521.

Melderis, A., 1978. Taxonomic notes on the tribe Triticeae(Gramineae) with special reference to the genera Elymus L. and Agropyron Gaertner. Bot. Jour. Linn. Soc. 76: 369-384.

Melderis, A., 1985. Agropyron (Gaertner). In: Davis PH (ed.), Flora of Turkey and the East Aegean Islands, Vol. 9, pp. 204-206. Edinburgh: Edinburgh University Press.

Melderis, A., Humpries, C. J., Tutin, T. G. and Heathcote, S. A., 1980. Tribe Triticeae Dumort. In: Tutin, T. G., Heywood, V. H., Burges, N. A., Moore, D. M., Valentine, D. H., Walters, S. M. and Webb, D. A. (eds.) Flora Europaea, Vol. 5, pp. 190-200. Cambridge University Press, Cambridge, England.

Mellish, A., Coulman, B. and Ferdinandez, Y., 2002. Genetic Relationships among Selected Crested Wheatgrass Cultivars and Species Determined on the Basis of AFLP Markers. Crop Sci. 42: 1662–1668.

Miller, M. P., 1997. AMOVA-PREP, a program for the preparation of AMOVA input files for use with WINAMOVA. Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ.

Mohamed, T. R., Khalifa, S. F. and Salah EL-Dine, R. M., 2006. Leaf Protein Electrophoretic Profiles and Chromosome Numbers of Some Araceae. Int. Jour. Agri. Biol. 8: 231–234.

Mueller, R. J. and Richards, J. H.,1986. Morphological Analysis of Tillering in Agropyron spicatum and Agropyron desertorum. Annal. Bot. 58:911–921.

Murphy, R. W., Sites, J. W., Buth, D. G. and Haufler, C. H., 1990. Protein I: Isozyme Electrophoresis. In: Hillis DH, Moritz C eds.Molecular Systematic.Sinnauer Association, Sunderland, Massachusetts. pp. 45–126.


Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 583–590.

Nevski, S. A., 1934. Tribe XIV. Hordeae Benth. In: V. L. Komorov (ed.) Flora of the USSR., pp. 590-722. Leningrad: The Botanical Institute of the Academy of Sciences of the USSR.

Paterson, A. H., Tanksley, S. D. and Sorreis, M. E., 1991. DNA markers in plant improvement. Adv. Agron. 46: 39-90.

Reddy, P. M. Munirajappa, M., 2005. Electrophoretic studies in induced mutants of diploid mulberry genotype S13. Indian Jour. Biotech. 4: 422–423.

Refoufi, A. and Esnault, M. A., 2008. Population genetic diversity in the polyploid complex wheatgrass using isoenzym and RAPD data. Biol. Plant. 52: 543-547.

Rogler, G. A. and Lorenz, R. J., 1983. Crested wheatgrass: Early history in the United States. Jour. Range Manage. 36: 91–93.

Salehi Shanjani, P., Jafari, A. A. and Calagari, M., 2013. Genetic variation among wild and cultivated Agropyron desertorum populations based on total protein profiles and phenotypic traits. New Zealand Jour. Crop Horticul. Sci. 41: 117-135.

Salehi Shanjani, P., Jafari, A. A. and Hoseinzadeh, R., 2013. Effects of selection on genetic parameters of Secale montanum based on seed storage protein marker. Jour. Rangeland Sci. 3: 231-244.

Taghizadeh, R., Jafari, A. A., Imani, A. A., Asghari, A. and Choukan, R., 2011. Investigation of genetic variability in Iranian populations of desert wheatgrass (Agropyron desertorum) based on morphological and RAPD markers. Iranian Jour. Rangelands For. Plant Breed. Genet. Res.  19: 85–100. (In Persian).

Tamura, K., Dudley, J., Nei, M. and kumar, S., 2007. MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596–1599.

Torkpo, S., Danquah, E., Offei, S. and Blay, E., 2006. Esterase, total protein and Seed storage protein diversity in Okra (Abelmoschus esculentus (L.) Moench). West African Jour. Applied Ecol. 9: 7–18.

Tzvelev, N. N., 1976. Tribe 3. TriticeaeDumort. In: Fedorov, A.A. (ed.), PoaceaeURSS. Navka Publishing House, Leningrad, pp. 147- 181.

Vries, I. M., 1996. Characterization and identification of Lactuca sativa cultivars and wild relatives with SDS–electrophoresis (Lactuca sect. Lactuca, Compositae). Genet. Resour. Crop Evol. 43: 193–202.

Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S.V., 1990. DNA polymorphisms amplified by arbitary primers are useful as genetic markers. Nucl. Acids Res. 18: 6531-6535.

Wright, S., 1931. Evolution in Mendelian populations. Genetics, 16: 97–159.

Wright, S., 1951. The genetical structure of populations. Annal. Eugentics, 15: 323–353.

Yeh, F. C., Yang, R. C. and Boyle, T., 1999. POPGENE version 1.32, Microsoft window base software for population genetic analysis: a quick user's guide. University of Alberta, Center for International Forestry Research, Alberta, Canada.

Yousofi, M. and Assadi, M., 2006. Tow New Taxa of Agropyron cristatum (Poaceae), for the flora of Iran. Iranian Jour. Botany, 12: 47-50. (In Persian).