Effect of Different Treatments on Improving Seed Germination Characteristics of Astragalus adscendens and Astragalus podolobus

Document Type : Research and Full Length Article


1 Natural Resources Faculty, University of Tehran

2 Watershed Management, Gorgan Agricultural and Natural Resources Sciences University, Gorgan

3 Watershed Management

4 Senior Expert of Research, Education and Extensions Organization of Jihade Agriculture Ministry, Tehran

5 Combating Desertification, Natural Resources Faculty, University of Tehran


Nowadays, in many rangelands, due to over grazing, and problems that some plants
have in germination, the rate of forage production is greatly reduced. So to take advantages of
the benefits of such plants, it is necessary to identify and remove barriers of germination and
establishment of suitable plants. The present study was designed to investigate different
treatments of breaking dormancy and stimulating seed germination of Astragalus adscendens
and Astragalus podolobus and offer the most effective treatment to enhance germination and
growth rate. The experiment was conducted using Completely Randomized Design (CRD)
with four replications. Treatments included hot water for 5 minute, 30% hydrogen peroxide for
5 minute, 98% sulfuric acid for 5 minute and chilling at 4°C for 10 days. Data were collected
for seed germination percentage, Germination Rate (GR) and Mean Germination Time
(MGT). Based on the results, treatment with 98% sulfuric acid for 5 min for A. adscendens
and hot water for 5 min for A. podolobus were the best treatments to break dormancy and
stimulate seed germination, so that the germination percentage increased from 44% to 82% in
A. adscendens and from 28% to 62% in A. podolobus. In A. adscendens 98% sulfuric acid
reduced the amount of MGT from 8 days for control treatment to 5 days. In A. podolobus hot
water treatment was more effective on MGT reduction and reduced it from 7 to 5 days.
Although sulfuric acid had the highest effect in breaking dormancy, but its application in a
vast scale is not easy, therefore the hot water was suggested as the substitution treatment.


Aliero, B. L., 2004. Effects of sulfuric acid, mechanical scarification and wet heat treatments on germination of seeds of African locust bean tree, Parkia biglobosa. Afr. Jour. Biotech, 3(3): 179-181.
Ansari, O., Sharifzadeh, F., Moradi, A., Azadi, M. S. and Younesi, E., 2013. Effect of heat shock treatment on germination characteristics of Secale montanum under aging conditions, Cercetari Agronomice in Moldova, 4(156): 21- 30. (In Persian).
Baskin, C. C. and Baskin, J. M., 1998. Seeds: Ecology, Biogeography, Evolution of Dormancy and Germination, Academic Press, San Diego. USA.
Ebrahimi-e-Rastaghi, M., 1981. Manna, production and utilization systems. Department of Natural Resources, Reported in Chaharmahal-o-Bakhtiari province, Iran. 75 pages. (In Persian).
Ellis, R. A. and Roberts, E. H., 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Tech., 9: 373-409.
Fateh, A., Majnoon Hosseini, N., Maddah Arefi, H. and Sharifzadeh, F., 2005. Methods of breaking dormancy in Astragalus tribuloides. Jour. Range. Forest. Plant Breed. Gen. Res., 13(4): 345-360. (In Persian).
Ghaderi, A., Kamkar, F. and Soltani, A., 2008. Seed science and technology (translation), Mashhad University Jihad Press, 512 pages, Mashhad, Iran. (In Persian).
Isvand, H., Maddah Arefi, H. and Tavakkol Afshari, R., 2005. Breaking of dormancy and germination of Astragalus siliquosus. Jour. Range. For. Plant Breed Gen. Res., 13(1): 67-84. (In Persian).
Jabarzare, A., Bassiri, M. and Yeganeh, H., 2010. Planting Methods and Seedling Establishment of Artemisia sieberi Besser: Seeds Collected from Isfahan Kolah Ghazi Sagebrush Vegetations, Jour. Range Sci, 1(1): 1-7. (In Persian).
Jouri, M. and Mahdavi, M., 2009. Applied identification of rangeland plants, Aij Press, Iran, 418 pages. (In Persian).
Levitt, J., 1974. Introduction to plant physiology .CV Mos by Company USA, Pp. 277-288.
Maddah Arefi, H., Nasiri, M. and Isvand, H., 2004. Investigation on changes of seed viability and breaking dormancy of some species in the gene bank of natural resources. Jour. Range. For. Plant Breed Gen. Res., 12(2): 163-182. (In Persian).
Masumi, A. A. and Ghahraman, A., 2006. Iran Astragals, Research Institute of Forests and Rangelands, 786 pages. (In Persian).
Tavili, A., Pouzesh, H., Farajolahi, A., Zare, S. and Zare Chahooki, M. A., 2010. The effect of different treatments on improving seed germination characteristics in medicinal species of Descurainia sophia and Plantago ovata. Afr. Jour. Biot., 9(39): 6588-6593. (In Persain).
Tavili, A., 2012. Rangeland plants seed technology, the text for M.Sc. course of Range Management, Natural Resources Faculty, University of Tehran. (In Persian).
Tran, V. and Cavanagh. A. K., 1984. Structural aspects of seed dormancy. In: Seed Physiology, Vol. 2, ed. D.R Murray. New York: Academic press.
Zare‚ S.‚ Tavili, A. and Darini, M. J., 2011. Effects of different treatments on seed germination and breaking seed dormancy of Prosopis koelziana and Prosopis juliflora. Jour. For. Res.‚ 22(1): 35-38. (In Persian).
Volume 4, Issue 2 - Serial Number 2
April 2014
Pages 110-117
  • Receive Date: 22 January 2014
  • Revise Date: 21 January 2014
  • Accept Date: 25 February 2014
  • First Publish Date: 25 February 2014