Effects of Silica and Silver Nanoparticles on Seed Germination Traits of Thymus kotschyanus in Laboratory Conditions

Document Type: Research and Full Length Article

Authors

1 Ph.D. Student of Range Management Science at the Department of Range & Watershed Management, University of Mohaghegh Ardabili, Ardabil, Iran

2 Associate Professor, University of Mohaghegh Ardabili, Ardabil, Iran

3 Assistant Professor, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

The introduction of nanoparticles into seed germination and seedling growth of plants might have a significant impact and thus, it can be used for agricultural applications for better growth and yield. The purpose of this study was to compare the effects of silica and silver nanoparticles on seed germination and early growth traits as well as percent and rate of germination, root length, shoot length, seedling fresh and dry weight and seed vigor index of Thymus kotschyanus. Experiment was conducted using a completely randomized design with four replications in winter 2014. Seed sources were from Sabalan rangelands, Ardabil province, Iran. Treatments were control (distilled water) and silica and silvernanoparticles with the concentration of 20 and 60%. Thirty seeds were sown in each Petri dish. Seed germination began from the fourth day after sowing and they were counted every day until germination was stopped. Seed germination was controlled for 14 days. The statistical analyses were conducted using analysis of variance (ANOVA). Duncan test was performed to examine the differences between the treatments. Results showed that the germination of T. kotschyanus was strongly affected by nano-silver treatments in comparison with nano-silica and control treatments. Overall, higher values of seed germination traits were observed in nano-silver (20%). Moreover, increasing silica nanoparticle concentration had enhanced the seed germination. In contrast, the increase of silver nanoparticle concentration had decreased the germination traits.

Keywords

Main Subjects


Adatia, M. H. and Besford, R. T., 1986. The effects of silicon on cucumber plants grown in recirculating nutrient solution. Annals of Botany, 58: 343-351.

Aghajantabar Alee, H., Pirdashti, H., Kashani, A. and Biparva, P., 2014. Effect of silver nanoparticles on germination of two plants Festuca ovina and Festuca arundinaceae stressed salinity. Jour. Range Management, 1: 33-45. (In Persian).

Azimi, R., Jankju Borzelabad, M., Feizi, H. and Azimi, A., 2014, Interaction of SiO nanoparticles with seed prechilling on germination and early seedling growth of tall wheatgrass (Agropyron elongatum L.). Polish Jour. Chemical Technology, 16(3): 25 -29.

Bao-shan, L., Shao-qi, D., Chun-hui, L., Li-jun, F., Shu-chun, Q. and Min, Y., 2004. Effects of TMS (nanostructured silicon dioxide) on growth of Changbai larch seedlings. Jour. Forestry Research, 15(2): 138-140.

Bekhrad, H., Mahdavi, B. and Rahimi, A., 2015. Effect of halopriming on germination, morphological and physiological characteristics of Sesamum indicum L. under alkalinity stress. Jour. Plant Production Researches. 22(2): 25- 46. (In Persian).

Cheng, L., Zheng, L., Li, G., Yao, Z., Yin, Q. and Jiang, K., 2008, Manufacture of epoxy-silica nanoparticle composites and characterisation of their dielectric behavior. International Jour. Nanoparticles, 1: 3–13.

Darvishi, L., Zare Chahouki, M. A., Jafari, M., Azarnivand, H. and Yousefi Valikchali, M., 2013. Study on the Environmental Factors Contributing to distribution of Thymus kotschyanus in Taleghan Basin, Iran. Jour. Rangeland Science, 4(1): 82- 90.

Ellis, R. H. and Roberts, E. H., 1981. The quantification of ageing and survival in orthodox
seeds. Seed Science and Technology, 9: 377-409.

El-Temsah, Y. S. and Joner, E. J., 2010. Impact of Fe and Ag nanoparticles on seed germination and differences in bioavailability during exposure in aqueous suspension and soil. Environmental Toxicology, 27(1): 42- 49.

Haghighi, M., Afifipour, Z. and Mozafarian, M., 2012. The Effect of N-Si on Tomato Seed Germination under Salinity Levels. Jour. Biological and Environmental Science, 6(16): 87-90.

Hong, F. S, Yang, F., Ma, Z. N., Zhou, J., Liu, C., Wu, C. and Yang, P., 2005. Influences of nano-TiO2 on the chloroplast ageing of spinach under light. Biological Trace Element Research, 104: 249–260.

Josko, I. and Oleszczuk, P., 2013. Influence of soil type and environmental conditions on ZnO, TiO2 and Ni nanoparticles phytotoxicity. Chemosphere, 92: 91–99.

Jyothsna, Y. and Pathipati, U., 2013. Environmental effects of nanosilver: impact on castor seed germination, seedling growth, and physiology. Environmental Science and Pollution Research, 20: 8636- 8648.

Karimi, N., Minaei, S., Almassi, M. and Shahverdi, A. R., 2012. Application of silver nanoparticles for protection of seeds in different soils. African Jour. Agricultural Research, 7(12): 1863-1869.

Khodakovskaya, M. V., Silva, K., Nedosekin, D. A., Dervishi, E., Biris, A. S., Shashkov, E. V., Ekaterina, I. G. and Zharov, V. P., 2011. Complex genetic, photo thermal, and photo coustic analysis of nanoparticle-plant interactions. Proceeding of Natural Academy Science, 108(3): 1028–1033.

Khorshidi, J., Rostaei, A. and Fakhre Tabatabaei, M., 2010. Effect of climate and phenological stage on essential oil quantity of Thymus caramanicus Jalas. Scientific Conference on Medicinal plant Industry Development in Iran. 28 February & 1 March, Tehran, Iran. (In Persian).

Klaus-Joerger, T., Joerger, R., Olsson, E. and Granqvist, C. G., 2001. Bacteria as workers in the living factory: metal-accumulating bacteria and their potential for materials science. Trends Biotechnology, 19(1): 15–20.

Lee, S. K., Sohn, E. Y., Hamayun, M., Yoon, L. Y. and Lee, I. J., 2010a. Effects of silicon on growth and salinity stress of soybean plant grown under hydroponic system. Agroforestry Systems, 80: 333-430.

Lee, W. L., Mahendra, S., Zodrow, K., Li, D., Tsai, Y. C., Braam, J. and Alvarez, P. J. J., 2010b. Developmental phytotoxicity of metal oxide nanoparticles to Arabidopsis thaliana. Environmental Toxicology and Chemistry, 29: 669–675.

Lee, W. M., An, Y. J., Yoon, H. and Kweon, H. S., 2008. Toxicity and bioavailability of copper nanoparticles to the terrestrial plants mungbean (Phaseolus radiatus) and wheat (Triticum aestivum): plant agar test for water-insoluble nanoparticles. Environmental Toxicology and Chemistry, 27(9): 1915–1921.

Lu, C. M., Zhang, C. Y., Wen, J. Q., Wu, G. R. and Tao, M. X., 2002. Research of the effect of nanometer materials on germination and growth enhancement of Glycine max and its mechanism. Soybean Science, 21: 168–172.

Ma, J. F., 2004. Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses. Jour. Soil Science and Plant Nutrition, 50: 11–18.

Majumder, D. D., Ulrichs, C., Mewis, I., Weishaupt, B., Majumder, D., Ghosh, A., Thakur, A. R., Brahmachary, R. L., Banerjee, R., Rahman, A., Debnath, N., Seth, D., Das, S., Roy, I., Sagar, P., Schulz, C., Linh, N. Q. and Goswami, A., 2007. Current status and future trends of Nano-scale technology and its impact on modern computing biology medicine and agricultural biotechnology. In: Proceedings of the international conference on computing: theory and applications. IEEE Press, ICCTA, India, March, 5–7: 563-572.

Manzer, H., Siddiqui, M. and Al-Whaibi, H., 2014. Role of nano-SiO2 in germination of tomato (Lycopersicum esculentum seeds Mill.). Saudian Jour. Biological Sciences, 21: 13–17.

Mazumdar, H., 2014. Accumulation and Uptake of Silver Nanoparticles during Seed germinations of selected annual crop plants. International Jour. Chem Tech Research. 6 (1): 108-113.

Monica, R.C., Cremonini, R., 2009. Nanoparticles and higher plants. Caryologia; International Journal of Cytology, Cytosystematics and Cytogenetics, 62: 161–165.

Musante, C. and White, J. C., 2012, Toxicity of Silver and Copper to Cucurbita pepo: Differential Effects of Nano and Bulk-Size Particles, Environmental Toxicology, 27(9): 510-517.

Panwar, P. and Bhardwaj, S. D., 2005. Hand book of practical forestry, Agrobios, India, 21p.

Parveen, A. and Rao, S., 2014. Effect of nano-silver on seed germination and seedling growth in Pennisetum glaucum. Jour. Cluster Science, 26 (3): 693-701.

Paulkumar, K., Arunachalam, R. and Annadurai, G., 2011. Biomedical applications of organically modified bioconjugated silica nanoparticles. International Jour. Nanotechnology, 8: 653-663.

Raskar, S. V. and Laware, S. L., 2014. Effect of zinc oxide nanoparticles on cytology and seed germination in onion. International Jour. Current Microbiology and Applied Sciences, 3 (2): 467-473.

Ratte, H. T., 1999. Bioaccumulation and toxicity of silver compounds: A review. Environmental Toxicology Chemistry, 18: 89–108.

Senapati, S., 2005. Biosynthesis and immobilization of nanoparticles and their applications. Ph.D. thesis, CSIR-National Chemical Laboratory, University of Pune, India.

Seyedi, M., Hamzei, J., Bourbour, A., Dadrasi, V. and Sadeghi, F., 2002. Effect of hydro-priming on germination properties and seedling growth of the safflower (Carthamus tinctorius L.) under drought stress. Jour. Agronomy Sciences, 5(8): 63-76.

Shahraki, M. R., Gholami Baghi, N., Sharafatmandrad, M. and Behmanesh, B., 2015. Rangelands Goods and Services Local People Views and Priorities (Case Study: Hezarjarib Rangelands, Mazandaran Province, Iran). Jour. Rangeland Science, 5(3): 212- 221.

Siddiqui, M. H. and Al-Whaibi, M. H., 2014. Role of nano-SiO2 in germination of tomato (Lycopersicum esculentum seeds. Mill.). Saudian Jour. Biological Sciences, 21: 13-17.

Singh, M., Shinjini, S., Prasad, S. and Gambhir, I. S., 2008. Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Digest Jour. Nanomaterials and Biostructures, 33:115-122.

Tan, X. M., Lin, C. and Fugetsu, B., 2009. Studies on toxicity of multiwalled carbon nanotubes on suspension rice cells. Carbon, 47: 3479-3487.

Tavili, A., Mirdashtvan, M., Alijani, R., Yousefi, M. and Zare, S., 2014. Effect of different treatments on improving seed germination characteristics of Astragalus adscendens and Astragalus podolobus. Jour. Rangeland Science, 4(2): 110-117.

Torney, F., Trewyn, B. G., Lin, V. S. Y. and Wang, K., 2007. Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nature Nanotechnology, 2: 295–300.

Ushahra, J., Bhati-Kushwaha, H. and Malik, C. P., 2014. Biogenic nanoparticle-Mediated augmentation of seed germination, growth, and antioxidant level of Eruca sativa Mill. Varieties. Applied Biochemistry Biotechnology, 174: 729–738.

Vashisth, A. and Nagarajan, S., 2010. Effect on germination and early growth characteristics in sunflower (Helianthus annuus) seeds exposed to static magnetic field. Jour. Plant Physiology, 167(2): 149–156.

Wang, X. D., Ou-yang, C., Fan, Z., Gao, S., Chen, F. and Tang, L., 2010. Effects of exogenous silicon on seed germination and antioxidant enzyme activities of Momordica charantia under salt stress. Jour. Animal and Plant Science, 6: 700-708.

Wang, X., Wei, Z., Liu, D. and Zhao, G., 2011. Effects of NaCl and silicon on activities of antioxidative enzymes in roots, shoots and leaves of alfalfa. African Jour. Biotechnology, 10: 545-549.

Wang, L. J., Guo, Z. M., Li, T. J. and Li, M., 2001. The nano structure SiO2 in the plants. Chinian Science Bulletin, 46: 625–631.

Yang, F., Hong, F. S., You, W. J., Liu, C., Gao, F. Q., Wu, C. and Yang, P., 2006. Influences of nano-anatase TiO2 on the nitrogen metabolism of growing spinach. Biological Trace Element Research, 110: 179–190.

Yin, L., Colman, B. P., McGill, B. M., Wright, J. P. and Bernhardt, E. S., 2012. Effects of silver nanoparticle exposure on germination and early growth of eleven wetland plants. PLOS ONE, 7(10): 1-7.

Zhu, H., Han, J., Xiao, J. Q. and Jin, Y., 2008. Uptake, translocation and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. Jour. Environmental Monitoring, 10: 713–717.

Zuccarini, P., 2008. Effects of silicon on photosynthesis, water relations and nutrient uptake of Phaseolus vulgaris under NaCl stress. Biologia Plantarum, 52: 157-160.