Investigating Allelopathic Effects of Artemisia sieberi on Seed Germination and Seedling Growth Indices of Three Alfalfa Species

Document Type : Research and Full Length Article


1 behbahan khatam alanbia university of technology

2 Assistant Professor, Department of Range and Watershed Management, Faculty of Natural Resources, Behbahan Khatam Alanbia University Of Technology


Allelopathy is one of the main factors limiting the plants growth. The present study investigated the effect of aqueous extract of leaves and fruit of Artemisia sieberi that is an important species for rangeland rehabilitation on seed germination and seedling growth of three alfalfa species (Medicago sativa cv. Nikshahri,‌ Medicago polymorpha and Medicago scutellata cv. Robinson) in laboratory and glasshouse. Treatments were five concentrations of 0, 25, 50, 75 100% Artemisia extract. Treatments were evaluated using a completely randomized factorial experiment in four replications in germinator and pots separately (2014). Data were collected and analyzed for the germination percent, germination rate, seedling length, seedling weight and seed vigor index. Means comparisons were made using Duncan test. The results showed significant differences between extract concentrations of Artemisia on all of traits in both growth conditions (P<0.05). There was a decrease trend for all of the traits. Germination percent of M. polymorpha was stopped in low concentration of Artemisia extracts (25%) whereas there were linear decreasing trends for the other specie until irrigation by 100% extraction. M. sativa had a higher germination rate and M. scutellata had higher vigor index values in all the treatments. Results of the study indicated that Artemisia had strong allelopathic effects and prevents from the germination and seedling growth of alfalfa.


Akramghaderi, F., Zeinali, E. and Farzaneh, S., 2001. Allelopathic effects of annual wormwood (Artemisia annua L.) on seedling emergence and growth of wheat, oil seed rape, wild mustard and wild oat. Jour. Agric. Sci. Natur. Resource, 8(3): 113-121. (In Persian)
Alipour, S.H., Fili Zadeh, Y. and Montazeri, M., 2010. Allelopathic Effects of Wormwood (Artemisia annua L.) on the Weeds of corn (Zea mays L.), Weed Research Jour., 2(1): 69-83. (In Persian)
Abdul– Baki, A.A. and Anderson, J.D., 1973. Vigor determination in soybean by multiple criteria. Crop Sci., 13: 630-633.
Asaduzzaman, M., An, M., Pratley, J. E., Luckett, D. J. and Lemerle, D., 2014. Canola (Brassica napus) germplasm shows variable allelopathic effects against annual ryegrass (Lolium rigidum), Plant and Soil, 380(1-2): 47-56.
Azarnivand, H. and Zare Chahouki, M. A., 2010. Range Improvement. Tehran University press 354pp. (In Persian).
Bagheri, R. and Mohammadi, S., 2011. Allelopathic effects of Artemisia sieberi Besser on three important species (Agropyron desertorum, Agropyron elongatum and Atriplex canescens) in range improvement, Iranian Jour. Range Desert Research, 17(4): 538-548. (In Persian)
Dehdari, S., Jafari, M., Hamedanian, F. and Tavili, A., 2008. Investigation on Allelopathic effects of Atriplex canescens (Fourwing sultbush) on seed germination of Salsola rigida, PajouheshVa Sazandgi, 81: 145-151. (In Persian)
Gholami, F., Dianati Tilaki, G.A. and Behtari, B., 2011a. Study of allelopathic effect of Artemisia herbaalba Asso. on seed germination and seedling growth of Onobrychis sativa L. and Medicago sativa L., Iranian Jour. Rangelands and Forests Plant Breeding and Genetic Research, 19 (1): 181-191. (In Persian)
Gholami, P. Ghorbani, J. and Ghaderi, Sh., 2011b. Allelopathic effects of Artemisia ausheri on seed germination and Dactylis glomerata properties of Festuca arundinacea Schreb, Jour. Plant Ecophysiology, 3(9): 42-52. (In Persian)
Inderjit, N. F. and Foy, C.L., 1999. Nature of the interference mechanism of Artemisia vulgaris, Weed Technology, 13(1): 176-182.
Jabbar Zare, A. and Basiri, M., 2009. Investigation of the allelopathic effects on seed germination extract of aerial parts of Artemisia sieberi, Jour. Rangeland, 3(4): 699-709. (In Persian).
Jouri, M.H. and Mahdavi, M., 2010. Applied identification of rangeland plants, Ayiizh Press, Tehran, Iran, pp. 192. (In Persian)
Kaur, S. Singh, H. P. Mittal, S. Batish, D. R. and Kohli, R. K., 2010. Phytotoxic effects of volatile oil from Artemisia scoparia against weeds and its possible use as a bio herbicide, Industrial Crops and Products, 32(1), 54-61.
Klyman, D.L., 1985. Qinghaosu (artemisin): an antimalaria drug from china, Science, 228: 1049-1055.
Lydon J., Teasdale, J.R. and Chen, P.K., 1997. Allelopathic activity of annual wormwood (Artemisia annua) and role of artemisin, Weed Sci., 45:807-811.
Macro, J.A. and Babera, O., 1990. Natural products from the gents Artemisia l, stud, Nat, Prod, 7: 201-264.
Maguire, J.D., 1962. Speed of germination- aid in selection and evaluation for seedling emergence and vigor. Crop Sci. 2: 176-177.
Maki Zadeh Tafti, M., Farhoodi, R., Rabiee, M. and Rasti Far, M., 2013. Effect of alcoholic extract of Artemisia annua on seed germination and growth of four weed species, Plant and Zistboom, 9(36): 101-109. (In Persian)
Meiners, S. J., 2014. Functional correlates of allelopathic potential in a successional plant community, Plant ecology, 215(6): 661-672.
Maighany, F., 2003. Allelopathy: From concept to application. Parto e Veghe, Tehran, Iran, 256 pp, (In Persian).
Mohebi, Z., Zare Chahouki, M. A. and Jafari, M., 2010. Allelopathic effects of Artemisia sieberi on seed germination and initial growth properties of Stipa barbata, Rangeland, 4(2): 298-307. (In Persian)
Molish, H., 1937. Der Einfluseiner Pflanze auf die andere, Allelopathie. Verlag von Gustav Fisher, Jena, Germany. English translation: La Fleur LJ, Mallik MAB (translators): Influence of one plant on another.
Nielsen, J. A., Frew, R. D., Whigam, P. A., Callaway, R. M. and Dickinson, K. J. M., 2015. Germination and growth responses of co-occurring grass pieces to soil from under invasive Thymus vulgaris, Allelopathy Jour., 35(1): 139-152.
Oliveira, A. K. Matias, R. Lopes, S. S. and Fontoura, F. M., 2014. Allelopathy and influence of leaves of Palicourea rigida (Rubiaceae) on seed germination and seedling formation in lettuce, Bioscience Jour., 30(5): 938- 947.
Oueslati, O., 2003. Allelopathy in two durum wheat (Triticum durum L.) varieties, Agric. Ecosys. Environ, 96: 161-163.
Preston, C.A. Betts, H. and Baldwin, I., 2002. Methyljasmonate as an allelopathic agent: Sagebrush inhibits germination of a Neighboring tobacco. Chemical Ecology, 28(11): 2343-2369
Rezai, M., Khajeddin, S.J and Safianian, A.R., 2007. Allelopathic effects of Scariola orientalis and Agropyron elongatum on Onobrychis viciaefolia, Jour. Rangeland, 4: 386-400, (In Persian).
Razmjui, D., Tavili, A., Jafari, M., Hanteh, A., Asareh, M.H. and Javadi, S.A., 2008. Comparison allelopathic effect Zataria multiflora on the characteristics of emergence and seedling growth of Stipa Arabica and Cymbopogon olivieri, Jour. Rangeland, 4: 421-435, (In Persian).
Rice, E.L., 1984. Allelopathy, 2nd Ed. Florida: Academic press 424 pp.
Saberi, M., Tavili, A. and Shahriari, A. R., 2012. The influence of chemical stimulators on decrease of Thymus kotschyanus allelopathic effect on Agropyron elongatum seed germination characteristics. Watershed Management Research (Pajouhesh & Sazandegi) 95: 45-54, (In Persian).
Sadeghi Shahrokht, T., Jangju, M., Nikbakht, Z. and Khavar, A., 2012. Investigate the seed culture Agropyron desertorum in the remaining limbs and the soil under the plant floor Peganum harmala L., Desert Ecosystems Engineering Jour., 1: 21-30, (In Persian).
Samedani, B. and Baghestani, M.A., 2005. Comparison of allelopathic activity of different Artemisia species on seed germination rate and seedling growth of Avena ludoviciana, Pajouhesh & Sazandegi, 68: 69-74. (In Persian).
Sedghi, M., GholiTolui, S. and Rezai, M., 2014. Allelopathic effect of (Dactylis glomerata L.) on germination and seedling growth of alfalfa, Jour. Plant Protection, 28(4): 532-538, (In Persian).
Shabani, G., Chaei Chi, M. R., TorkNegad, A., Heidari Sharif Abad, H. and EshghiZadeh, H. R., 2004. The effects of time and environmental conditions on the percentage of hard seed breakdown in annual medic (Medicago scutellata) cv. Robinson, Pajeuhesh & Sazandegi, 63: 91-95, (In Persian).      
Tabatabaee Zade, M.S., Pajouhan, M., Soltani, M., Tajamolian, M. and Shahbandari, R., 2014. Allelopathic Effects of Artemisia aucheri boiss Essential Oils on Seed Germination and Early Seeding Growth of Red-root Amaranth, (Amaranthus retroflexus L.) and Field Bindweed (Convolvlus arvensis L.) Knowledge of sustainable agricultural production, 24(3): 87- 95, (In Persian).
Tavili, A. Jannat Rostami, M. and Ebrahimi Dorcheh, K.H., 2009. Inhibitory effects of Artemisia sieberi on germination properties of Salsola rigida, Iranian Jour. Range Desert Research, 16(6): 409-418, (In Persian).
Torabi Asl, S. Rohani, H. Gholam Alipoor Alamdari, E. and Tahmasebi, A., 2013. Study of allelopathic potential of some rangeland species Artemisia sieberi Besser on germination, physiological and biochemical Agropyron elongatum (Host) Beauv, Jour. Plant Ecosystem Conservation, 1(2): 31-42, (In Persian).
Turk, M.A. and Tawaha, A.M., 2003. Allelopathic effect of black mustard (Brassica nigra L.) on germination and growth of wild oat (Avena fatua L.). Jour. Crop Protection, 22: 673-677.             
Yang, X. S. Deng, R. De., Philippis, L., Chen, C. Hu, and Zhang, Chemical, W., 2012. Composition of volatile oil from Artemisia ordosica and its allelopathic effects on desert soil microalgae, Palmellococcus miniatus. Plant Physiology and Biochemistry, 51: 153-158.
Yun, K.W. and Han, D.M., 1993. Phytotoxic and antimicrobial activity of volatile constituents of Artemisia princeps var. orientalis. Ecological Modeling, 19: 2757-2766.