Dynamic Changes of Main Rumen Microflora and Ruminal Fermentation in Sheep Supplemented with Molasses-Urea

Document Type: Research and Full Length Article


1 Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences

2 Faculty of Life and Environment Science, University of Tsukuba


The digestion and utilization of roughage by sheep depends on rumen digestion. The aim of this study was to evaluate the microbe quantity and fermentative efficiency of rumen supplement with molasses-urea. This experiment was conducted in 2014, eight sheep were selected and divided into two groups (a control group and a treatment group), and only the treatment group animals were supplied with molasses-urea for ad libitum consumption. Rumen fluid was collected every 2 h and rumen fermentation parameters were measured. The populations of majority bacteria were investigated by real-time PCR. The results showed that the populations of majority bacteria increased in the rumens of treatment group animals (P<0.05). Each bacterium quantity decreased gradually after feeding, and reached the lowest level 2 h after intake. It then slowly increased and reached the highest level at 8 h after intake. Finally, each bacterium quantity returned to the same level as before intake. In contrast, the protozoa number raised to the highest at 4 h after intake and declined gradually. The concentration of protozoa in the treatment group sheep was significantly higher than that of control group (P<0.05). The pH of rumen liquids was found in a normal range and was not different between both groups. However, the pH decreased from the highest level before feeding to the lowest level within 4 h, and it increased after intake for 8 h. The concentration of NH3-N and microbial crude protein (MCP) synthesis, in the rumen liquids, were both significantly higher than that of control group (P<0.05), the highest concentration of NH3-N and MCP was reached after feeding by 2 h and 4 h, respectively. Molasses-urea has a positive effect on the rumen, due to their favorable effect on rumen fermentation by the microbes in ruminant.


Main Subjects

Broderick, GA. and Craig, WM., 1989. Metabolism of peptides and amino acids during in vitro protein degradation by mixed rumen organisms. Jour. Dairy Sci., 72: 2540-2548.

Cotta, MA. and Russell, JB., 1982. Effect of peptides and amino acids on efficiency of rumen bacterial protein synthesis in continuous culture. Jour. Dairy Sci., 65: 226-234.

Dehority, BA., Tirabasso, PA. and Grifo, AP., 1989. Most-probable-number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium. Applied and Environmental Microbiology, 55(11): 2789-2792.

Deng, ZF., Ouyang, XX., Liu, HX. and Kang, H., 2008. The effect test of different added levels of goats urea blocks. Acta Ecologiae Animalis Domastici, 29(5): 93-95.

Emmanuel, N., Patil, NV., Bhagwat, SR., Lateef, A., Xu, K. and Liu, HN., 2015. Effects of different levels of urea supplementation on nutrient intake and growth performance in growing camels fed roughage based complete pellet diets. Animal Nutrition, 4:356-361.

Greenwood, RH., Titgemeyer, EC. and Drouillard, JS., 2000. Effects of base ingredient in cooked molasses blocks on intake and digestion of prairie hay by beef steers. Jour. Anim. Sci., 78: 167-172.

Koike, S., Kobayshi, Y., 2001. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiology Letters, 204: 361-366.

Kostenbauder, MJ., Coleman, SW., Chase, Jr CC., Kunkle, WE., Hall, MB. and Martin, FG., 2007. Intake and digestibility of bahia grass hay by cattle that are supplemented with molasses or molasses-urea with or without soybean hulls. The Professional Animal Scientist, 23:373–380.

Leedle, JA., Bryant, MP. and Hespell, RB., 1982. Diurnal variations in bacterial numbers and fluid parameters in ruminal contents of animals feed low-or high-forage diets. Applied and Environmental Microbiology, 44: 402-412.

Li, CQ., Alatengdalai, Xue SY., Tajimaa, A. and Ishikawa, N., 2015. Estimation of herbage intake and digestibility of grazing sheep in Zhenglan Banner of Inner Mongolia by using n-alkanes. Animal Nutrition, 1(4): 324-328.

Li, D., Wang, JQ., Bu, DP., Yang, SL., Wei, HY. and Zhou, LY.. 2008. Determination of the effects of soybean oil and linseed oil in diets on the quantities of rumen cellulolytic bacteria in beef cattle by real-time PCR. Chinese Jour. Animal Nutrition, 20(3): 256-260.

Li, SZ., Gao, FJ., Yang, YY., Jiang, N., Zhao, YN., Li, YH., Yang, HM. and Ji, H., 2011. Effects of glutamine and l-carnitine on ruminal cellulolytic bacteria of sheep under low temperatures. Chinese Jour. Animal Nutrition, 23(03): 499-505.

Meng, Q., Kerley, MS. and Belyen, R L., 1995. Effect of dilution rate and fermentable structural polysaccharide to protein ratio on microbial efficiency in continuous culture. Jour. Anim Sci, 73 (Suppl, 1): 262.

Tajima, K., Aminov, RI., Nagamine, T., Matsui, H., Nakamura, M. and Benno, Y., 2001. Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Applied and Environmental Microbiology, 67(6): 2766-2744.

Wan, GD., Li, CL., Pan, ZR., Wang, YP. and Duan, GS., 2009. Effect of urea-molasses nutrient block on sheep in cold season. Pratacultural Science, 26(1):123-125.

Wang, HR., Chen, XW. and Wang, MZ., 2011. Effect of Yucca schidigera saponin and tea saponin mixture on the rumen fermentation and its fibrolytic bacterial activity in the rusitec substrates with different concentrate to forage ratio, Scientia Agricultura Sinica, 44(8): 1710-1719.

Wang, HR. and Zhang, JR., 2011. Effect of different dietary carbohydrate structure on fermentation and amino acid profile of microorganisms in the rumen of goats. Scientia Agricultura Sinica, 44(5):1071-1076.

Wang, SX., Yin, SF., Zheng, JL., 2010. Study on fattening effect of beef cattle with different amounts of molasses. China Cattle Science, 36(1): 32-35.

Yang, SL., Wang, JQ., Hu, ZY., Li, D. and Sun, HX., 2007. Effects of soybean oil and linseed oil supplementation on the rumen fermentation and main rumen microflora of cattle. Scientia Agricultura Sinica, 40(10): 2316-2322.

Zhang, DG., 1998. Supplementary feeding on urea molasses multinutrient blocks and effects on productive performance of yak cows. Acta Prataculturae Sinica, 7(01):65-69.

Zhang, WJ., Li, SL., Shi, HT. and Luo, YP., 2013. Different kinds of molasses: effects on feed intake and milk performance of dairy cows during summer heat stress. Chinese Jour. Animal Nutrition, 25(1): 163-170.