Nutritive Value and Digestibility of Rumex obtusifolius in Three Phenological Stages by Chemical, Nylon Bag and Gas Production Methods

Document Type: Research and Full Length Article


1 Department of animal Sciences, Faculty of Agriculture and Natural resources, Sanandaj branch, Islamic Azad University, Sanandaj, Iran

2 M.Sc. (Graduated), Department of Animal Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

3 Department of Animal Sciences, Faculty of Agriculture, Rasht Branch, Islamic Azad University, Rasht, Iran


The present study was conducted to identify nutritional value of Rumex obtusifolius in three phenological stages (vegetative growth, flowering, and maturity). Samples were randomly taken in pastures of Saravan in Rasht, Iran in 2015. After drying and grinding, the chemical compositions of Crude Protein (CP), Crude Fiber (CF), Ether Extract (EE), Total Ash (ASH), Neutral Detergent Fiber (NDF), and Acid Detergent Fiber (ADF) were measured based on standard methods. Gas production (in vitro) and Dry Matter (DM) degradability (in sacco) were determined. Phenological stage effects were studied using a completely randomized design in three replications and data were analyzed using SAS software. In phenological stages of the plant with the progress of maturity, the amounts of CP, ASH, Nitrogen Free Extract (NFE), and Non Fibrous Carbohydrates (NFC) decreased while the CF, NDF, and ADF contents increased. CP had the highest value (23.92%) in the vegetative growth and lowest value (6.11%) in maturity stage. The gas production in 96 hours of vegetative growth, flowering, and maturity stages was 37.68, 40.76, and 26.69 ml/0.2g of dry matter. The DM degradability in 96 hours for vegetative growth, flowering, and maturity stages was 80.25, 70.42, and 40.43%, respectively. The correlation coefficient between gas production method and DM degradability methods were 0.99, 0.98, and 0.97 for vegetative growth, flowering, and maturity stages, respectively. Therefore, the gas production method due to its lower cost was suggested for measuring nutritive value of this plant. The results showed that considering higher or equal protein content of this plant during the vegetative growth and flowering stages as compared to alfalfa, it can be used for ruminant animal diet. Using this wild source is possible to significantly decrease the expenses involved in animal nourishment.


Main Subjects

Adesogan, A.T., 2002. What are feeds worth? A critical evaluation of selected nutritive value methods. Proceeding 13th annual Florida ruminant nutrition symposium. University of Florida, Gainesville, USA, 33-47 pp.

Akbarzadeh, A., Jaimand, K., Hemmati, A. and Khanjani Shiraz, B., 2010. Medicinal plants of Gilan province and their applications, Iranian Jour. Medicinal and Aromatic Plants, 26: 326-347. (In Persian).

AOAC, 2000.Official methods of analysis of the AOAC International, 17th Edition, Gaithersburg, MD: AOAC International.

Arzani, H., Basiri, M., Khatibi, F. and Ghorbani, G., 2006. Nutritive value of some Zagros mountain rangeland species, Small Ruminant Research, 65: 128-135. (In Persian).

Arzani, H., Zohdi, M., fisher, E., Zahedi Amiri, G.H., Nikkhah, A. and Waster, D., 2004. Phenological effects on forage quality of five grass species. Jour. Range Management, 57: 624-630. (In Persian).

Blummel, M. and Becker, K., 1997. The degradability characteristics of fifty-four roughages and roughage neutral detergent fibers as described by in vitro gas production and their relationship to voluntary feed intake. British Jour. Nutrition, 77: 757-768.

Blümmel, M. and Orskov, E.R., 1993. Comparison of In vitro gas production and nylon bag degradability of roughages in predicting feed intake in cattle. Animal Feed Science and Technology, 40:109-119.

Canbolat, O., Kamalak, A., Zkan, E.O., Erol, A., Sabin, M., Karakas, E. and Ozkose, E., 2006. Prediction of relative feed value of alfalfa hays harvested at different maturity stages using in vitro gas production. Livestock Research and Rural Development, 18 (2): 1-7.

Crowder, L.V., 1985. Pasture management for optimum ruminant production, In: Nutrition of grazing ruminants in warm climates, Edited by: L.R., McDowell, Academic Press, INC.

De Boever, J.L., Aerts, J.M., Vanacker, J.M. and De Brander, D.L., 2005. Evaluation of the nutritive value of maize silages using a gas production technique. Animal Feed Science and Technology, 255: 123-124.

Foster, J.L., Muir, J.P., Lambert, B.D. and Pawelelk, D., 2007. In situ and in vitro degradation of native Texas warm- season legumes and alfalfa in steers fed a sorghum- Sudan basal diet. Animal Feed Technology, 133: 228- 239.

Ghanbari, A. and Sahraei, M., 2012. Determination of nutritional value in three forage species in three phonological stages in Sabalan rangelands, Ardebil, Iran. Jour. Rangeland Science, 2: 449-457, (In Persian).

Getachew, G., Blummel, M., Makkar, H.P.S. and Becker, K., 1998. In vitro gas measuring techniques for assessment of thenutritional quality of feeds: a review. Animal Feed Science and Technology, 72: 261-269.

Hajipuor, S., Barani, H., Yeganeh, H. and Abdei Sarvestani, A., 2017. Factors affecting herders migration time to summer rangelands (case study: Kouhdasht rangelands, Lorestan. Jour. Rangeland Science, 7: 199-209.

Heady, H.F. and Dennis-Child, R., 1994. Rangeland ecology and management. Weest View Press, U.S.A., pages: 530.

Hejduk, S. and Dolezal, P., 2004. Nutritive value of broad-leaved dock (Rumex obtusifolius L.) and its effect on the quality of grass silage. Czech Jour. Animal Sciences, 49: 144–150.

Holchek, J.L., Wofford, H., Artgun, D., Galyean, M.L. and Wallace, J.D., 1986. Evaluation of total fecal collection for measuring cattle forages. Jour. Range Management, 1: 39-45.

Honěk, A. and Martinková, Z., 2002. Effects of individual plant phe­nology on dormancy of Rumex obtusifolius seeds at dispersal. Weed Research, 42: 148–155.

Kamalak, A., Canbolat, O., Gurbuz, Y. and Ozay, O., 2005. Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation. Czech Jour. Animal Sciences, 50: 60- 67.

Khanum, S.A., Yaqoob, T., Sadaf, S., Hussain, M., Jabbar, M.A, Hussain, H.N, Kausar, R. and Rehman, S., 2007. Nutritional evaluation of various feedstuffs for livestock production using in vitro gas method. Pakistan Veterinary Jour., 27: 129-133.

Khazaal, K., Markantonatos, X., Nastis, A. and Orskov, E.R., 1993. Changes with maturity in fiber composition and levels of extractable polyphenols in Greek browse: effect in vitro gas production and in saccodry matter degradation. Jour. Science and Food Agriculture, 63: 237-244.

Kleinschmit, D.H., Schingoethe, D.J., Hippen, A.R. and Kalscheur, K.F., 2007. Dried distillers grains plus soluble with corn silage or alfalfa hay as the primary forage source in dairy cow diets. Jour. Dairy Sciences, 90: 5587- 5599.

Kone, J.W. and Van Geldor, H.A., 1999. Influence of protein fermentation of gas production profiles. Animal Feed Science and Technology, 76: 128- 251.

Lee, M.J., Hwang, S.Y. and Chiou, P.W.S., 2000. Metabolizable energy of roughage in Taiwan. Small Ruminant Research, 36: 251-259.

Mahala, A.G., Fadel, E. and Abdel Nasir, M.A., 2007. Chemical composition and in vitro gas production characteristics of six fodder trees leaves and seeds. Research Jour. Agriculture and Biological Sciences, 3: 983-986.

Menke, K.H., Rabb, L., Saleweski, A., Steingass, H., Fritz, D. and Schinder, W., 1979. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. Jour. Agricultural Sciences, 93: 217- 222.

Menke, K.H. and Steingass, H., 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development, 28: 7- 55.

Ndlovu, L.R. and Nherera, F.V., 1997. Chemical composition and relationship to in vitro gas production of Zimbabwean browsable indigenous tree species. Animal Feed Science and Technology, 69: 121-129.

NRC, 2001. Nutrient Requirements of Dairy Cattle. 7th ed. Nat. Acad. Sci., Washington, DC.

Orskov, E.R., 1989. Recent advances in the evaluation of roughages as feeds for ruminants. In: Farell D.J. (ed): Advances in animal nutrition. University of New England Printery, Armidale, 102-108 pp.

Orskov, E.R. and McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to the rate of passage. Journal of Agricultural Sciences, 92: 499- 503.

Pinkerton, B., 1996. Forage quality. Crop and soil environment Science College of agriculture. Forest and life science, Clemson University, 65-76 pp.

Ramirez, R.G., Gonzalez, H., Morales, R., Cerrillo, A., Julrez, A., Garsia, G.J. and Guerrero, M., 2009. Chemical composition and dry matter digestion of some native and cultivated grasses in Mexico. Jour. Animal Sciences, 54: 150-162.

Rezaeian, M., Chaudhry, A.S. and Honarzad, J., 2006. Nutrient composition and in vitro organic matter degradability of desert plants from Iran. Proceedings of the British Society of Animal Science, March, 2006, Midlothian, UK.

Rhodes, D.A. and Sharrow, S.H., 1990. Effect of grazing by sheep on the quantity and quality of forage available to thebig game in Oregon coast range. Jour. Range Management, 43: 235- 237.

Riasi, A., Danesh Mesgaran, M., Stern, M.D. and Ruiz Moreno, M.J., 2008. Chemical composition, in situ ruminal degradability and post-ruminal disappearance of dry matter and crude protein from the halophytic plants Kochia scoparia, Atriplex dimorphostegia, Suaeda arcuataand Gamanthus gamacarpus. Animal Feed Science and Technology, 141: 209-219.

Saravani, M., Shahraki, E. and HormoziPur, H., 2013. A study on the nutritional value of the plant species Lamb'stongue (Plantago lanceolata), European bindweed (Convolvulus arvensis), and Annual sowthistle (Sonchus oleraceus). International Jour. Agriculture and Crop Sciences, 6: 808-813. (In Persian).

SAS, 2003. SAS Users Guide. SAS Institute Inc. Cary, NC.

Songsak, C., Anut, C. and Piyante, C., 2007. Chemical compositions and nutritional evaluation of energy feeds for ruminant using in vitro gas production technique. Pakistan Jour. Nutrition, 6: 607-612.

Taghizadeh, A., A., Safamerh, V., Palangi and Mehmannavaz, Y., 2008. The determination of metabolizable protein in some feedstuffs used in ruminant. Research Jour. Biological Sciences, 3: 804-806. (In Persian).

Turgut, L., Yanar, M., Tuzemen, N. and Comakli, B., 2008. Effect of maturity stage on chemical composition in situ ruminal degradability kinetics of meadow hay in Awassi sheep. Jour. Animal and Veterinary Advances, 7: 1061-1065.

Vahdani, N., Moravej, H., Rezayazdi, K. and Dehghan-Banadaki, M., 2014. Evaluation of Nutritive Value of Grass Pea Hay in Sheep Nutrition and Its Palatability as Compared with Alfalfa. Jour. Agricultural Science and Technology, 16: 537-550. (In Persian).

Van Soest, P.J., 1994. Nutritional ecology of ruminants. 2nd ed. Cornell University Press, USA.

Van Soest, P.J., Robertson, J.B. and Lewis, B.A., 1991. Methods for dietary fiber neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. Jour. Dairy Sciences, 74: 3583- 3597.

Vanzant, E.S., Cochran, R.C. and Titgemeyer, E.C., 1998. Standardization of in situ techniques for ruminant feedstuff evaluation. Jour. Animal Science, 76: 2717–2729.