The Effects of Seed Priming with Acid Ascorbic on Seed Germination and Morphological Traits of Taverniera cuneifolia under Drought Stress

Document Type : Research and Full Length Article


1 Range Management Department, Faculty of Natural Resources, Tarbiat Modares University,

2 Tarbiat Modares University, Faculty of Natural Resources

3 Center of Natural Resources and Agriculture, Mazandaran


The species of Taverniera cuneifolia belonging to the family of legominoseae is a perennial species usually growing in the south of Iran. The objective of this study was to evaluate the effects of the drought stress and seed priming using acid ascorbic (AsA) on seed germination of Taverniera cuneifolia in laboratory and seedling morphological traits in greenhouse conditions. This study was conducted in 2015 in Iran. In laboratory, a factorial experiment was conducted using Polyethylene glycol 6000 in drought stress (0, -4, -8, -16 bar) and priming AsA in four levels (0, 50, 100 and 200 mM) for 24 hours and in the greenhouse, experiment AsA in four levels (0, 50, 100 and 200 mM) and drought stress in four irrigation periods (3, 6, 9 and 12 days). Experimental design was a completely randomized design in four replications with 30 seeds per pots per replication. In greenhouse, seeds were sown in plastic pots in soil. All pots were irrigated until germination stage. Then, drought stresses were applied for 64 days and finally, morphological traits were measured. The data were analyzed and means comparisons were made using Duncan test (P<0.05). Results showed that increasing drought stress caused significant decreases in the seed germination, and morphological traits of T. cuneifolia. The lowest seed germination, shoot length, root length and vigor index were obtained in drought stress of -16 bar. The highest germination rate was obtained in priming seeds in 200 mM AsA and non drought stress treatment. The highest specific leaf area (cm2/g), leaf area (cm2), and dry weight biomass were found in priming seeds in 200 mM AsA. In conclusion, priming with AsA improved the seed germination rate and morphological traits in seedling of T. cuneifolia.


Main Subjects

Abdul-Baki, A.A., Anderson, J.D., 1973. Vigor determination in soybean seed by multiple criteria. Crop Sci. 13, 630-633.
Akhondi, M., Safarnejhad, A., Lahoti, M., 2004. Indices morphology of genotypes of (Medicago sativa) against drought stress, Pajouhesh & sazandegi, 62(1): 51-57,(In Persian).
Arias, D., Calvo-Alvarodo, J., Dohrenbusch, A., 2007. Calibration of LAI -2000 to Estimate Leaf Area Index and Assessment of its Relationship with stand productivity in six Native and Introduced tree species in costarica, Forest Ecology and management, 247(1-3): 185-193.
Ashraf M., Foolad M. R., 2005. Pre-Sowing Seed Treatment-A Shotgun Approach to Improve Germination Plant Growth and Crop Yield under Saline and None-Saline Conditions, Advances in Agronomy, 88(1): 223-271.
Bahreininejad B., Razmjoo J., Mirza M., 2013. Influence of water stress on morpho-physiological and phytochemical traits in Thymus daenensis, International Journal of Plant Production, 7(1):151-166.‏
Beltagi M. S., 2008. Exogenous ascorbic acid (vitamin C) induced anabolic changes for salt tolerance in chick pea (Cicer arietinum L.) plants, African Journal of Plant Science, 2(10): 118-123.‏
Bradford KJ. 1986. Manipulation of seed water relation via osmotic priming to improve germination under stress conditions. Hort Sci 21: 1105-1112.
Cantliffe, D.J. 2003. Seed Enhancements. Acta Horticulturae 607: 53-59.
Demir Kaya, M., Okcu, G., Atak, M., Cikili, Y., Kolsarici, O., 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Europ. Jour. Agronomy. 24, 291-295.
Demir, I., Mavi, K., 2004.The effect of priming on seedling emergence of differentially matured watermelon (Citrullus lanatus (Thunb.) Matsum and Nakai) seeds. Scientia Horticulture. 102, 467-473.
Dianati Tilaki, Gh. A., Shakarami, B., Tabari, M. 2011. Alleviation of salinity stress on the germination and early growth of three fescue species with seed priming treatments, Propagation of Ornamental Plants, 11(2): 102-108.
Dianati Tilaki, G. A., Behtari, B., Alizadeh, M. A., Ashraf, A., 2010. Effect of Seed Priming on Germination and Seedling Growth of Festuca Arundinacea Schreb and Agropyron Desertorum (Fisch. ex link) JA Schultes, Povolzhskiy Journal of Ecology, 26(3): 323-330.
Dianati Tilaki, G. A., Behtari, B., 2009. Effect of salt and water stress on the germination of alfalfa (Medicago sativa L.) seed, Povolzhskiy Journal of Ecology, 2(1): 158-164.
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., Basra, S.M. 2009. Plant drought stress: effects, mechanisms and management. Agronomy Sustainable Development, 29: 185–212.
Gairola, K.C., Nautiyal, A.R., Dwivedi, A.K. 2011.Effect of Temperatures andGermination Media on Seed Germination of Jatropha curcas Linn, Advances in Bioresearch, 2(2): 66-71.
Hoekstra, F.A., Golovina, E.A., Hemminga, E.A. 1999, Imbibitional leakage from anhydrobiotes revisited, Plant Cell and Environment, 22(9): 1043-1175.
ISTA., 2003. International Seed Testing Association. ISTA Handbook on Seedling Evaluation. 3rd ed. Bassersdorf, Switzerland.
Masoumi Zavariyan, A., Yousefi Rad,M., Asghari, M., 2015. Effect of seed priming with kinetin on germination indices and proline activity of Silybum marianum L. under drought stress conditions, International Journal of Life Sciences,.9(1): 23-28.
Mohammadi, K., Moghadam, A. K., Aghaalikhani, M., & Vaziri, M. 2014. Effect of Hydro-priming and Priming with Ascorbic and Salicylic Acid on Germination Traits of Dracocephalum moldavica L. Varieties, Journal ofEssential Oil Bearing Plants, 17(5): 936-943.
Mukherjee, M., Larrimore, K. E., Ahmed, N. J., Bedick, T. S., Barghouthi, N. T., Traw, M. B., Barth, C., 2010. Ascorbic acid deficiency in arabidopsis induces constitutive priming that is dependent on hydrogen peroxide, salicylic acid, and the NPR1 Gene, The American Phytopathological Society, 23(3): 340–351.
Murillo‐Amador, B., López‐Aguilar, R., Kaya, C., Larrinaga‐Mayoral, J., Flores‐Hernández, A., 2002. Comparative effects of NaCl and polyethylene glycol on germination, emergence and seedling growth of cowpea, Journal of Agronomy and Crop Science, 188(4): 235-247.‏
Naik, V.N., 1998. Flora of Marathwada (Ranunculaceae to Convolvulaceae). Amrut Prakashan, Aurangabad, India.
Nonami, H., 1998: Plant water relations and control of cell elongation at low water potentials. Journal of Plant Research 111: 373–382.
Passam, H.C. and Kakouriotis D. 1994. The effects of osmo-conditioning on the germination, emergence and early plant growth of cucumber under saline conditions. Scientia Horticulturae, 57: 23-240.
Powell, A.A., L.J. Yule, H.C. Jingh and P.C. Groots. 2000. The influence of aerated hydration seed treatment on seed longevity as assessed by the viability equation. Journal of Experimental Botany 51: 2031-2043.
Sadat noori, A., Khalaj, H., Shiranirad, A. H., Alahdadi, I., Akbari, GH. A., Labafi Hasan Abadi, M. R., 2015. Investigation of seed vigour and germination of canola cultivars under less irrigation in padding stage and after it, PakistanJournal of Biological Science, 10(7): 2880-2884.
Schonfeld, M.A., Johnson, R.C., Carver, B.F.,.Mornhinweg, D.W., 1988. Water relations in winter wheat as drought resistance indicator. Crop Science,28: 526–531.
Senaratna, T., D. Touchell, E. Bumm and K. Sixon. 2000. Acetyl salicylic and salicylic acid induced multiple stress tolerance in bean and tomato plants. Plant Growth Regulation 30: 157-161.
Stadler, M., Dagne, E., Anke, H., 1994. Nematicidal activity of two phytoalexins from Taverniera abyssynica. Planta Med. 60 (6), 550-552.
Taiz, L., Zeiger, E., 2010: Plant Physiology, 5th ed. Sinauer Associates Inc Publishers, Sunderland, MA, USA.
Taylor, A.G., P.S. Allen, M.A. Bennett, K.J. Bradford, J.S. Burris and M.K. Misra. 1998. Seed enhancement. Seed Science Research 8: 245-256.
Wiebe, H.J. and H. Tiesses. 1979. Effects of different seed treatments on embryo growth and emergence of carrot seeds. Gartenbauwissenschaft 44: 280-286.
  • Receive Date: 04 April 2017
  • Revise Date: 12 August 2017
  • Accept Date: 14 April 2017
  • First Publish Date: 01 July 2018