Investigation Phenol, Flavonoids and Antioxidant Activity Content of Capparis spinosa in Three Natural Habitats of Sistan and Baluchestan Province, Iran

Document Type : Research and Full Length Article

Authors

1 Range and Watershed Management Department, Water and Soil Collage, University of Zabol

2 Assistant Professor, Rangeland Research Division, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

3 Assistant Professor, Department of Range and Watershed Management, Faculty of Agriculture and Natural Resources, Lorestan University, Khorranabad, Iran

4 Assistant Professor, Desert Studies Faculty, Semnan University

Abstract

Capparis spinosa L. is a shrub plant that in addition to its forage use, has protective importance to prevent soil erosion in desert areas and importance values in treating many diseases as well. The aim of this study was to investigate the amount of phenol, flavonoids and antioxidant activity in different organs of C. spinosa in Sistan, Iranshahr and Saravan counties, Iran. Morphological traits (number of fruits, wet weight of fruit, dry weight of fruit, fruit diameter, number and length of branches, plant height, leaf length, leaf width and root depth) in each habitat were measured from four individuals of C. spinosa randomly. In order to perform phytochemical tests, different parts of the plant (stem, leaves, flowers, fruits, and roots) were randomly collected from the habitats in the post-flowering stage in June 2019. The total phenol and flavonoid content of all methanolic extracts were measured using the spectrophotometric method and antioxidant activity was determined using the free radical trap method. Data analysis was performed as factorial experiment based on completely randomized design in four replications. Rresults significant differences between different plant organs (P<0.01) in aspect of the antioxidant activity, the amount of total phenol and flavonoids. Also, there was a significant plant organs by habitats interaction (P<0.01). The results of the mean comparison showed the highest total phenol and total flavonoids were obtained from the methanolic extract of the flower 82.8 mg of quercetin equivalent per gram dry weight and 64.3 mg of gallic acid/g of dry weight in Sistan region, respectively, and the highest antioxidant activity was 15.7% in the fruit in Iranshahr region. According to the results, the obtained methanolic extract of C. spinosa flower and fruit in Sistan natural habitats is recommended to the treatment of diseases as a potential source of natural antioxidants.

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Volume 12, Issue 2
April 2022
Pages 191-204
  • Receive Date: 24 October 2020
  • Revise Date: 02 June 2021
  • Accept Date: 04 July 2021
  • First Publish Date: 04 July 2021